Predict functional sites
PreDom:Evtrace predicts functional sites of a protein on the basis of phylogenetic and structural information.
- Homologous proteins of the target protein are collected by homology search with BLAST.
- A phylogenetic tree is constructed from the multiple alignment of the sequences of the homologous proteins by the NJ method.
- Sequence clustering is performed on the phylogenetic tree, and then conserved residues and class-specific residues (TR residues) are extracted.
- Surface regions in which the TR residues are concentrated are identified as canditates of functional regions.