N-BIO BINDS

Predict functional sites

PreDom:Evtrace predicts functional sites of a protein on the basis of phylogenetic and structural information.

  1. Homologous proteins of the target protein are collected by homology search with BLAST.
  2. A phylogenetic tree is constructed from the multiple alignment of the sequences of the homologous proteins by the NJ method.
  3. Sequence clustering is performed on the phylogenetic tree, and then conserved residues and class-specific residues (TR residues) are extracted.
  4. Surface regions in which the TR residues are concentrated are identified as canditates of functional regions.

Search for homologous sequences
by BLAST
>>
Homologous sequences to
query data
>>
Results of evtrace
  • [input]
  • [input type] dataid
  • [database] UniProtKB/Swiss-Prot
  • [E-value] 0.01
  • [identity threshold] 20
  • [coverage threshold] 90
  • [multiple alignment method] clustalW2
Input
BLAST
parameters
Database: E-value: